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cytomegalovirus cmv pp65 peptide pools  (Miltenyi Biotec)


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    Miltenyi Biotec cytomegalovirus cmv pp65 peptide pools
    (A) Representative flow plots and (B) summary data for the frequency of IFN-γ-producing CD56 dim NK cells following 6-hour of stimulation with antibody-coated Raji cells (Target cells) in individuals with subtype A and non-A subtype infection. Control = Uncoated cells. (C) Representative flow plots and (D) pie charts showing IFN-γ production in FcεRγ + CD57 + CD56 dim and FcεRγ − CD57 + CD56 dim NK cells in participants with subtype A and non-A subtype infection. (E) The frequencies of IFN-γ + FcεRγ − CD57 + CD56 dim NK cells (dashed line) and log 10 viral loads (grey solid line) at all time points. (F) Representative plots for the identification of antigen-reactive NK cells based on double expression (IFN-γ and CD107a) following 6-h stimulation with media alone (control) or with HIV-1 (Gag and Env) or CMV <t>(pp65)</t> peptide pools. (G) a Percentage of responders (subtype A) to HIV-1 peptide pools at each time point or CMV pools at 1-month time point. (H) Frequency of HIV-reactive NK cells at all time points. Pie charts representing the proportion of antigen-reactive NK cells within adaptive (NKG2C + ) or canonical (NKG2C − ) NK cells. Significance determined by two-tailed Mann– Whitney U test or Wilcoxon matched-paired signed rank test. *p < 0.05, **p < 0.01. The non-parametric Spearman test was used for correlation analysis (two-tailed).
    Cytomegalovirus Cmv Pp65 Peptide Pools, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmv+pp65+peptide+pool/PepTivator+CMV+pp65%2C+premium+grade/bio_rxiv__2025__03__17__643703-263-24-28
    Average 95 stars, based on 98 article reviews
    cytomegalovirus cmv pp65 peptide pools - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "An early functional adaptive NK cell signature drives optimal CD8 + T-cell activation and predicts sustained HIV-1 viral control"

    Article Title: An early functional adaptive NK cell signature drives optimal CD8 + T-cell activation and predicts sustained HIV-1 viral control

    Journal: bioRxiv

    doi: 10.1101/2025.03.17.643703

    (A) Representative flow plots and (B) summary data for the frequency of IFN-γ-producing CD56 dim NK cells following 6-hour of stimulation with antibody-coated Raji cells (Target cells) in individuals with subtype A and non-A subtype infection. Control = Uncoated cells. (C) Representative flow plots and (D) pie charts showing IFN-γ production in FcεRγ + CD57 + CD56 dim and FcεRγ − CD57 + CD56 dim NK cells in participants with subtype A and non-A subtype infection. (E) The frequencies of IFN-γ + FcεRγ − CD57 + CD56 dim NK cells (dashed line) and log 10 viral loads (grey solid line) at all time points. (F) Representative plots for the identification of antigen-reactive NK cells based on double expression (IFN-γ and CD107a) following 6-h stimulation with media alone (control) or with HIV-1 (Gag and Env) or CMV (pp65) peptide pools. (G) a Percentage of responders (subtype A) to HIV-1 peptide pools at each time point or CMV pools at 1-month time point. (H) Frequency of HIV-reactive NK cells at all time points. Pie charts representing the proportion of antigen-reactive NK cells within adaptive (NKG2C + ) or canonical (NKG2C − ) NK cells. Significance determined by two-tailed Mann– Whitney U test or Wilcoxon matched-paired signed rank test. *p < 0.05, **p < 0.01. The non-parametric Spearman test was used for correlation analysis (two-tailed).
    Figure Legend Snippet: (A) Representative flow plots and (B) summary data for the frequency of IFN-γ-producing CD56 dim NK cells following 6-hour of stimulation with antibody-coated Raji cells (Target cells) in individuals with subtype A and non-A subtype infection. Control = Uncoated cells. (C) Representative flow plots and (D) pie charts showing IFN-γ production in FcεRγ + CD57 + CD56 dim and FcεRγ − CD57 + CD56 dim NK cells in participants with subtype A and non-A subtype infection. (E) The frequencies of IFN-γ + FcεRγ − CD57 + CD56 dim NK cells (dashed line) and log 10 viral loads (grey solid line) at all time points. (F) Representative plots for the identification of antigen-reactive NK cells based on double expression (IFN-γ and CD107a) following 6-h stimulation with media alone (control) or with HIV-1 (Gag and Env) or CMV (pp65) peptide pools. (G) a Percentage of responders (subtype A) to HIV-1 peptide pools at each time point or CMV pools at 1-month time point. (H) Frequency of HIV-reactive NK cells at all time points. Pie charts representing the proportion of antigen-reactive NK cells within adaptive (NKG2C + ) or canonical (NKG2C − ) NK cells. Significance determined by two-tailed Mann– Whitney U test or Wilcoxon matched-paired signed rank test. *p < 0.05, **p < 0.01. The non-parametric Spearman test was used for correlation analysis (two-tailed).

    Techniques Used: Infection, Control, Expressing, Two Tailed Test, MANN-WHITNEY

    Related Articles

    other:

    Article Title: Dysregulated NFκB-dependent ICOSL Expression in Human Dendritic Cell Vaccines Impairs T-cell Responses in Melanoma Patients
    Article Snippet: The cells were then washed and split into three groups: negative control (mDCs alone), IgG-treated control (10 μg/mL; eBioscience, Catalog # 16-4714-82), or an anti-ICOSL-treated group (10 μg/mL; eBioscience, Catalog #16-5889-82).

    Concentration Assay:

    Article Title: Massively parallel single cell analysis
    Article Snippet: .. For CMV stimulation, 1 ml of whole blood was stimulated with CMV pp65 peptide pool diluted in PBS (Miltenyi Biotec) at a final concentration of 1.81 μg/ml for 6 hours at 37 C. A separate aliquot of whole blood of each donor was incubated with PBS as negative controls. .. CD8+ T cells were isolated using RosetteSep cocktail (StemCell) and subsequently deposited onto microwell arrays.

    Article Title: Characterization of a Cytomegalovirus-Specific T Lymphocyte Product Obtained Through a Rapid and Scalable Production Process for Use in Adoptive Immunotherapy.
    Article Snippet: Phenotype was analyzed using FlowJo software v10. .. Cryopreserved expanded cells were stimulated with CMV pp65 peptide pool (Miltenyi) 50 ng/mL at a cell concentration of 1E+06 cells/mL in a 96-well plate. ..

    Incubation:

    Article Title: Massively parallel single cell analysis
    Article Snippet: .. For CMV stimulation, 1 ml of whole blood was stimulated with CMV pp65 peptide pool diluted in PBS (Miltenyi Biotec) at a final concentration of 1.81 μg/ml for 6 hours at 37 C. A separate aliquot of whole blood of each donor was incubated with PBS as negative controls. .. CD8+ T cells were isolated using RosetteSep cocktail (StemCell) and subsequently deposited onto microwell arrays.

    Negative Control:

    Article Title: Divergent SARS-CoV-2-specific T- and B-cell responses in severe but not mild COVID-19 patients.
    Article Snippet: .. Unstimulated cells, with no added peptides, was used a negative control and CMV pp65 peptide pool (100ng/ml, PepTivator CMV pp65, Miltenyi) was used for comparison. .. Activation with soluble αCD3 (Thermofisher, clone HIT3A) was used as a positive control and unstimulated cells (no addition of peptides) where used as negative control.

    Comparison:

    Article Title: Divergent SARS-CoV-2-specific T- and B-cell responses in severe but not mild COVID-19 patients.
    Article Snippet: .. Unstimulated cells, with no added peptides, was used a negative control and CMV pp65 peptide pool (100ng/ml, PepTivator CMV pp65, Miltenyi) was used for comparison. .. Activation with soluble αCD3 (Thermofisher, clone HIT3A) was used as a positive control and unstimulated cells (no addition of peptides) where used as negative control.

    Blocking Assay:

    Article Title: Cytotoxic CD8 + Temra cells show loss of chromatin accessibility at genes associated with T cell activation.
    Article Snippet: PBMC-s were thawed in X-Vivo 15 cell medium (Lonza). .. After washing, mix of diluted costimulants [anti-CD28 (BD) and anti-49d (BD)], pure CD40 antibody (for blocking CD40-CD154 binding; frontiersin.org Miltenyi Biotec) and also CMV pp65 peptide pool (1 mg of each peptide per ml, PepTivator, Miltenyi Biotec) were added to each sample. ..

    Article Title: Cytotoxic CD8 + Temra cells show loss of chromatin accessibility at genes associated with T cell activation
    Article Snippet: PBMC-s were thawed in X-Vivo 15 cell medium (Lonza). .. After washing, mix of diluted costimulants [anti-CD28 (BD) and anti-49d (BD)], pure CD40 antibody (for blocking CD40-CD154 binding; Miltenyi Biotec) and also CMV pp65 peptide pool (1 μg of each peptide per ml, PepTivator, Miltenyi Biotec) were added to each sample. ..

    Binding Assay:

    Article Title: Cytotoxic CD8 + Temra cells show loss of chromatin accessibility at genes associated with T cell activation.
    Article Snippet: PBMC-s were thawed in X-Vivo 15 cell medium (Lonza). .. After washing, mix of diluted costimulants [anti-CD28 (BD) and anti-49d (BD)], pure CD40 antibody (for blocking CD40-CD154 binding; frontiersin.org Miltenyi Biotec) and also CMV pp65 peptide pool (1 mg of each peptide per ml, PepTivator, Miltenyi Biotec) were added to each sample. ..

    Article Title: Cytotoxic CD8 + Temra cells show loss of chromatin accessibility at genes associated with T cell activation
    Article Snippet: PBMC-s were thawed in X-Vivo 15 cell medium (Lonza). .. After washing, mix of diluted costimulants [anti-CD28 (BD) and anti-49d (BD)], pure CD40 antibody (for blocking CD40-CD154 binding; Miltenyi Biotec) and also CMV pp65 peptide pool (1 μg of each peptide per ml, PepTivator, Miltenyi Biotec) were added to each sample. ..



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    (A) Representative flow plots and (B) summary data for the frequency of IFN-γ-producing CD56 dim NK cells following 6-hour of stimulation with antibody-coated Raji cells (Target cells) in individuals with subtype A and non-A subtype infection. Control = Uncoated cells. (C) Representative flow plots and (D) pie charts showing IFN-γ production in FcεRγ + CD57 + CD56 dim and FcεRγ − CD57 + CD56 dim NK cells in participants with subtype A and non-A subtype infection. (E) The frequencies of IFN-γ + FcεRγ − CD57 + CD56 dim NK cells (dashed line) and log 10 viral loads (grey solid line) at all time points. (F) Representative plots for the identification of antigen-reactive NK cells based on double expression (IFN-γ and CD107a) following 6-h stimulation with media alone (control) or with HIV-1 (Gag and Env) or CMV <t>(pp65)</t> peptide pools. (G) a Percentage of responders (subtype A) to HIV-1 peptide pools at each time point or CMV pools at 1-month time point. (H) Frequency of HIV-reactive NK cells at all time points. Pie charts representing the proportion of antigen-reactive NK cells within adaptive (NKG2C + ) or canonical (NKG2C − ) NK cells. Significance determined by two-tailed Mann– Whitney U test or Wilcoxon matched-paired signed rank test. *p < 0.05, **p < 0.01. The non-parametric Spearman test was used for correlation analysis (two-tailed).
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    Image Search Results


    (A) Representative flow plots and (B) summary data for the frequency of IFN-γ-producing CD56 dim NK cells following 6-hour of stimulation with antibody-coated Raji cells (Target cells) in individuals with subtype A and non-A subtype infection. Control = Uncoated cells. (C) Representative flow plots and (D) pie charts showing IFN-γ production in FcεRγ + CD57 + CD56 dim and FcεRγ − CD57 + CD56 dim NK cells in participants with subtype A and non-A subtype infection. (E) The frequencies of IFN-γ + FcεRγ − CD57 + CD56 dim NK cells (dashed line) and log 10 viral loads (grey solid line) at all time points. (F) Representative plots for the identification of antigen-reactive NK cells based on double expression (IFN-γ and CD107a) following 6-h stimulation with media alone (control) or with HIV-1 (Gag and Env) or CMV (pp65) peptide pools. (G) a Percentage of responders (subtype A) to HIV-1 peptide pools at each time point or CMV pools at 1-month time point. (H) Frequency of HIV-reactive NK cells at all time points. Pie charts representing the proportion of antigen-reactive NK cells within adaptive (NKG2C + ) or canonical (NKG2C − ) NK cells. Significance determined by two-tailed Mann– Whitney U test or Wilcoxon matched-paired signed rank test. *p < 0.05, **p < 0.01. The non-parametric Spearman test was used for correlation analysis (two-tailed).

    Journal: bioRxiv

    Article Title: An early functional adaptive NK cell signature drives optimal CD8 + T-cell activation and predicts sustained HIV-1 viral control

    doi: 10.1101/2025.03.17.643703

    Figure Lengend Snippet: (A) Representative flow plots and (B) summary data for the frequency of IFN-γ-producing CD56 dim NK cells following 6-hour of stimulation with antibody-coated Raji cells (Target cells) in individuals with subtype A and non-A subtype infection. Control = Uncoated cells. (C) Representative flow plots and (D) pie charts showing IFN-γ production in FcεRγ + CD57 + CD56 dim and FcεRγ − CD57 + CD56 dim NK cells in participants with subtype A and non-A subtype infection. (E) The frequencies of IFN-γ + FcεRγ − CD57 + CD56 dim NK cells (dashed line) and log 10 viral loads (grey solid line) at all time points. (F) Representative plots for the identification of antigen-reactive NK cells based on double expression (IFN-γ and CD107a) following 6-h stimulation with media alone (control) or with HIV-1 (Gag and Env) or CMV (pp65) peptide pools. (G) a Percentage of responders (subtype A) to HIV-1 peptide pools at each time point or CMV pools at 1-month time point. (H) Frequency of HIV-reactive NK cells at all time points. Pie charts representing the proportion of antigen-reactive NK cells within adaptive (NKG2C + ) or canonical (NKG2C − ) NK cells. Significance determined by two-tailed Mann– Whitney U test or Wilcoxon matched-paired signed rank test. *p < 0.05, **p < 0.01. The non-parametric Spearman test was used for correlation analysis (two-tailed).

    Article Snippet: After overnight rest, PBMCs were stimulated for 6 hours with 2 μg/ml of cohort-specific HIV-1 Gag or ENV peptide pools (provided by IAVI) or cytomegalovirus (CMV)-pp65 peptide pools (Miltenyibiotec) in the presence of αCD28/αCD49d co-Stim antibodies (1 μg/ml), GolgiStop (containing Monensin, 2 μmol/l), GolgiPlug (containing brefeldin A, 10 μg/ml) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences, Catalog # 562623, dilution 1 in 200).

    Techniques: Infection, Control, Expressing, Two Tailed Test, MANN-WHITNEY